Many laboratories still rely on either: Hot-shot lysis Fast and inexpensive, but originally designed to get DNA into a PCR reaction, not to deliver the consistency required for sequencing workflows.

 

Linearly downscaled extraction kits. Reducing every reagent volume lowers costs, but also reduces binding capacity, increases pipetting sensitivity and can compromise yield and purity. Both approaches solved yesterday's challenge:

 

Keeping cost per sample low. Today's challenge is different. Producing consistent DNA quality for GBS, SNP genotyping and sequencing.